| IL-2Rα

The Interleukin 2 Receptor Alpha (also called Tac antigen, P55, and mainly CD25) is a protein involved in the assembly of the high-affinity interleukin-2 receptor, consisting of alpha (IL2RA), beta (IL2RB) and the common gamma chain (IL2RG). This receptor interacts with interleukin-2, a pleiotropic cytokine which plays an important role in immune homeostasis. Interleukin-2 receptor alpha chain is an integral-membrane protein, more precisely type I transmembrane protein. This bitopic polypeptide is constructed by a sequence of 272 amino acids and has a molecular mass of around 3.8 kDa.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	20	10	5	2.5	1.25	0.62	0.31	0
OD	2.56	1.84	1.22	0.81	0.47	0.26	0.15	0.07
Corrected OD	2.49	1.77	1.15	0.74	0.4	0.19	0.08	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Mouse IL-2Rα spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	83-101	96
Plasma(n=8)	92-105	102
Cell Culture Supernates(n=8)	97-108	105

 

| Linearity

Samples were spiked with high concentrations of Mouse IL-2Rα and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	82-95	88-96	90-110
	Average (%)	91	93	96
1 : 4	Range (%)	88-103	87-108	106-115
	Average (%)	94	98	108